Journal: Frontiers in Immunology

Article Title: Deficient IL-2 Produced by Activated CD56 + T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection

doi: 10.3389/fimmu.2019.01647

Figure Lengend Snippet: Phenotypic characteristics of NK cells after incubation with L-2, IL-15, IFN-α, and IFN-β. (A) Comparison of CD16, CD69, and CD161 expressions on NK cells from the healthy donors after incubation with IL-2, IL-15, IFN-α, and IFN-β (50 ng/ml, 12 h), respectively ( n = 8). (B) Images of CD3, CD56, CD16, CD69, and CD161 expressions on CD56 dim NK cells at single-cell level after incubation with IL-2, IL-15, IFN-α, and IFN-β (50 ng/ml, 12 h), respectively. Data is acquired in an ImageStream x Mark II system. All P -values are two-tailed and significantly different with P < 0.05.

Article Snippet: All samples were stained with anti-CD3 eFluor 450 (ebioscience, clone UCHT1), anti-CD16 APC-Cy7 (BD, clone 3G8), anti-CD56 PE-Cy7 (BD, clone B159), anti-CD69 APC (BD, clone FN50), and anti-CD161 PE-Cy5 (BD, clone DX12).

Techniques: Incubation, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Deficient IL-2 Produced by Activated CD56 + T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection

doi: 10.3389/fimmu.2019.01647

Figure Lengend Snippet: IL-2 produced by CD56 + T cells facilitates NK-ADCC response. (A) Representative flow plots of IFN-γ secretion and CD107a expression of purified NK cells and of NK cells from bulk PBMCs with the stimulation of Ab-opsonized P815 cells. (B) IFN-γ secretion and CD107a expression of purified NK cells were lower than that of NK cells from bulk PBMCs ( n = 10) during ADCC response. (C) Secretion of IL-2 during ADCC response. PBMCs were stimulated by P815, Ab-opsonized P815 cells or PI (PMA+ionomycin) for 12 h at 37°C. Culture supernatants were collected and IL-2 level was detected by ELISA. Cell pellets were also collected and IL-2 mRNA level in PBMCs was detected by qRT-PCR ( n = 10). (D) Representative flow plots of IL-2 and CD107a expressions on CD56 − T cells, CD56 + T cells and NK cells with the stimulation of P815 cells only, Ab-opsonized P815 cells or PI. (E) Comparison of the abilities of IL-2 secretion and CD107a expression among CD56 − T cells, CD56 + T cells and NK cells with different stimulators ( n = 10). (F) Representative flow plots of IL-2 and CD107a expressions on CD56 − T cells, CD56 + T cells and NK cells with the HIV-1 elite plasma in the presence or absence of gp120 protein antigen. (G) Comparison of the abilities of IL-2 secretion and CD107a expression among CD56 − T cells, CD56 + T cells and NK cells with different stimulators ( n = 5). All P -values are two-tailed and significantly different when the value is <0.05.

Article Snippet: All samples were stained with anti-CD3 eFluor 450 (ebioscience, clone UCHT1), anti-CD16 APC-Cy7 (BD, clone 3G8), anti-CD56 PE-Cy7 (BD, clone B159), anti-CD69 APC (BD, clone FN50), and anti-CD161 PE-Cy5 (BD, clone DX12).

Techniques: Produced, Expressing, Purification, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Deficient IL-2 Produced by Activated CD56 + T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection

doi: 10.3389/fimmu.2019.01647

Figure Lengend Snippet: Dysfunction of CD56 + T cells contributes to the impaired NK-ADCC response in chronic HIV subjects. (A) Representative flow plots of lymphocytes in PBMC and CD56 + T depleting lymphocytes sorted by BD FACS Aria III. (B) Comparison of the abilities of CD107a and IFN-γ expression of NK cells from lymphocytes in PBMC and CD56 + T depleting lymphocytes ( n = 5). (C) Comparison of CD107a expression of NK cells in the present or absent of anti-IL-2 antibody during the process of ADCC response. ADCC response is triggered by Ab-opsonized P815 cells ( n = 10). (D) Purified NK cells were cultured with or without IL-2 antibody and/or CD56 + T cells (transwell insert) ( n = 10). Ab-opsonized P815 cells were added to all wells (top and bottom) to trigger the ADCC response. The frequencies of CD107a+ NK cells between groups were compared. (E) Comparison of frequencies of CD56+ T cells in lymphocytes between HIV-1-infected patients ( n = 50) and healthy controls ( n = 30). (F) Comparison of the abilities of IL-2 secretion from CD56 + T between HIV-1-infected patients ( n = 50) and healthy controls ( n = 30), with the stimulations of Ab-opsonized P815 cells or P815 cells alone. Data is shown as mean ± SD. All P -values are two-tailed and significantly different when P -value is < 0.05.

Article Snippet: All samples were stained with anti-CD3 eFluor 450 (ebioscience, clone UCHT1), anti-CD16 APC-Cy7 (BD, clone 3G8), anti-CD56 PE-Cy7 (BD, clone B159), anti-CD69 APC (BD, clone FN50), and anti-CD161 PE-Cy5 (BD, clone DX12).

Techniques: Expressing, Purification, Cell Culture, Infection, Two Tailed Test

Journal: Frontiers in Immunology

Article Title: Deficient IL-2 Produced by Activated CD56 + T Cells Contributes to Impaired NK Cell-Mediated ADCC Function in Chronic HIV-1 Infection

doi: 10.3389/fimmu.2019.01647

Figure Lengend Snippet: Schematic diagram of interaction between NK cells and CD56 + T cells during the process of HIV-1-specific ADCC immune response.

Article Snippet: All samples were stained with anti-CD3 eFluor 450 (ebioscience, clone UCHT1), anti-CD16 APC-Cy7 (BD, clone 3G8), anti-CD56 PE-Cy7 (BD, clone B159), anti-CD69 APC (BD, clone FN50), and anti-CD161 PE-Cy5 (BD, clone DX12).

Techniques: